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The method is based on the detection of presence of free carbonyl c=o group of reducing sugars Includes reagents, preparation, and procedure. At the end of the process, measure the absorbance of reducing sugar released in the reaction at 530 nm and calculate the enzyme activity using standard curve of reducing xylose.
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Read extinction at 540 mm against the blank Learn the dinitrosalicylic acid (dns) method for estimating reducing sugar concentration Please note that all the tubes must be cooled to room temperature before reading, since the absorbance is sensitive to temperature.
This method is based on the reaction of reducing sugars with dns reagent, which results in the formation of a coloured compound that can be measured spectrophotometrically.
The reaction was stopped by adding 1 ml dnsa and boiling, so this volume addition is not relevant Similarly, the subsequent addition of 9 ml of water is not relevant.
